HPLC-DAD同时分析甘草中7种有效成分

Simultaneous Analysis of Seven Active Components in Glycyrrhiza uralensis Fisch by HPLC-DAD

目的采用HPLC—DAD中梯度洗脱和检测波长时间序列采样的方法，建立甘草（Glycyrrhiza uralensis Fisch．）中4种黄酮成分（甘草苷、异甘草苷、甘草素、异甘草素）和3种甘草皂苷成分（甘草酸、甘草皂苷G2、乌拉尔甘草皂苷B）的同时分析方法。方法采用C18（4．6mm×250mm，5um）色谱柱。流动相：乙腈-（0．5％醋酸铵＋1％冰醋酸），梯度洗脱0～12min从6：94到22：78，12～25min从22：78到25：75，25—45min从25：75到40：60，45～50min从40：60到6：94，保持5min；检测波长：时间序列采样。结果7种成分的线性关系良好，平均加样回收率在98％～103％之间。结论该方法准确、灵敏度高，可用于甘草药材质量的评价。

OBJECTIVE To establish the method for simultaneous analysis of four flavone components （ liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin） and three saponin components （glycyrrhizin, licorice-saponin G2 and uralsaponin B） in Glycyrrhiza uralensis Fisch. using gradient elution and timetable detected wavelength by HPLC-DAD. METHODS The chromatographic separation was performed on a C18 （4. 6 mm×250 mm,5 um） column eluted with a mobile phase of aeetonitril-（0. 5% NH4Ac ＋ 1% HAc ） , gradient program used was as follows ：0 - 12 min 6：94 linear change to 22：78,12 -25 min 22：78 linear change to 25：75,25 - 45 rain 25：75 linear change to 40：60,45 -50 min 40：60 linear change to 6： 94, and this was maintained for 5 min and time-table detected wavelength. RESULTS Seven components had nice linear relation between the peak area and injected concentration. The average recoveries of the seven components were in the range from 98% to 103%. CONCLUSION The method was accurate, and sensitive. It＇s suitable to determine seven active components in G. uralensis and can be used for evaluating the quality of G. uralensis.