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Real-time PCR法定量检测柑橘绿霉病菌对抑霉唑的抗性频率 被引量：5

Real-time PCR assay for detection of the frequency of imazalil-resistance of Penicil-lium digitatum
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摘要 抑霉唑被广泛用来防治由指状青霉菌(Penicillium digitatum)引起的柑橘绿霉病。已有研究表明,柑橘绿霉病菌对抑霉唑的抗性由CYP51基因启动子区5个126-bp转录增强子的简单串联重复和126-bp转录增强子上199-bp的特异性片段插入所引起。基于这2种抗性分子机制,通过设计特异引物和优化条件,建立real-time PCR高通量分子检测技术,用于快速检测柑橘包装贮藏库中绿霉病菌群体对抑霉唑的抗性频率FR,指导科学用药。 Imazalil is a systemic fungicide used worldwide for the control of citrus green mold caused by Penicillium digitatum. Up to date, two molecular mechanisms of resistance to the fungicide imazalil were found from P. digitatum isolates in Zhejiang province. One was five copies of a 126-bp transcriptional enhancer unit in promoter region of CYP51 gene （resistant type I ） and the other was a unique 199-bp insert within the 126bp transcriptional enhancer unit （resistant type Ⅱ ）. Based on these mechanisms, a real-time PCR assay was developed to rapidly quantify the frequency of the resistance （ FR ） of P. digitatum in pathogen samples collected from storehouses of citrus.
作者 陈广进 张志芳 姜丽英 徐发三 马忠华 李红叶
机构地区 浙江大学农业与生物技术学院生物技术研究所 浙江省衢州市农业信息和培训中心 浙江省衢州市植物保护站
出处 《植物病理学报》 CAS CSCD 北大核心 2008年第6期561-569,共9页 Acta Phytopathologica Sinica
基金 浙江省科技厅科技项目资助(2008C32027 2007C22007) 国家“863”计划(2007AA102422)
关键词 柑橘绿霉病 抑霉唑 杀菌剂抗性 REAL-TIME PCR PeniciUium digitatum imazalil fungicide resistance real-time PCR
分类号 S666 [农业科学—果树学] S481.4 [农业科学—农药学]
  • 相关文献

参考文献10

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共引文献113

同被引文献71

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二级引证文献80

植物病理学报
2008年 第6期

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