尖锐湿疣皮损中朗格汉斯细胞功能状态的研究

Study on functional states of Langerhans cells distributed in lesions of condyloma acuminatum

目的通过分析尖锐湿疣(condyloma acuminatum,CA)皮损中郎格尔汉斯细胞(Langerhanscell,LC)的特征性表型,探讨其在CA微环境中的功能状态。方法30例初发CA皮肤组织为标本,20例正常包皮为对照标本。应用免疫组化技术检测标本中LC的CD1a、HLA-DR、S-100蛋白的表达,采用Motic彩色医学图文分析系统以及光学显微镜进行定量、半定量分析,并对不同蛋白分子标记的LC数量进行相关性分析。结果(1)Motic彩色医学图文分析系统以及光学显微镜分析结果显示:与正常对照组相比,尖锐湿疣皮损中CD1a(+)LC数量、表面标志物蛋白表达密度差异无统计学意义(P>0.05);而S-100(+)LC、HLA-DR(+)LC数量、蛋白表达密度则减少,差异有统计学意义(P<0.05);S-100(+)LC/CD1a(+)LC比值为0.082±0.061,与对照组相比差异显著(P<0.05)。(2)CA皮损表皮中CD1a(+)LC、S-100(+)LC、HLA-DR(+)LC的平均灰度值相关性分析结果显示;CA皮损表皮中LC上CD1a与HLA-DR的表达呈显著正相关;CD1a(+)LC、S-100(+)LC以及S-100(+)LC、HLA-DR(+)LC两者之间无线性相关性。结论CA皮损中存在不同标记的LC,这些不同标记的LC的数量和其表面标记蛋白的表达密度和正常对照组相比存在差异,提示:CA微环境中LC功能异常是HPV致病的可能机制之一。

Objective To explore the functional status of Langerhans cells （LC） in the skin lesions of condyloma acuminatum（CA） via analyzing the characteristic phenotype of the LCs. Methods 30 cases of primary CA were used as the testing samples, while 20 cases normal human foreskin tissues were taken as controls. LC surface protein markers including CD1a, S-100 and HLA-DR were analyzed by immunohistochemistry assay. Quantitative and semi-quantitative results of these protein markers were taken via Motic colorful medical map system and visional microscope observation. Relative analysis was investigated between the numbers of LCs with different protein markers. Results （1） Assayed by Motic colorful medical map system and visional microscope observation, it was showed that for CD1a（＋） LC, there were no significant differences of cell numbers and the protein marker densities on the cell surface between CA group and the control one （P〉0.05）, but for S-100（＋） LCs and HLA-DR（＋） LCs, the corresponding cell numbers and protein densities were decreased significantly compared the CA group with the controls （P〈0.05）. The cell number ratio of S-100 （＋） LC/CD1a （＋） LC is 0.082：1：0.061, which showed significance compared with the controls （P〈0.05）. （2） By analyzing the average gray scale on different LCs surface which presents the markers expression level, it was concluded that there was a linear relativity between LCs with protein expression of CD 1 a and HLA-DR in epidermis of CA group. However, there was no linear relationship between CD1a （＋）LC and S-100 （＋） LC, as well as S-100 （＋） LC and HLA-DR （＋） LC. Conclusions There exist various types of LCs according to their protein markers which present different development phases. The numbers of the various LCs and the densities of the protein markers on the cells in the CA lesion are more different compared with normal control. Our research suggested that aberrant function states of the LCs in the CA micro-environment might be the potent pathological factor for HPV infection.