摘要
目的了解酶免法手工检测与全自动检测HBsAg结果之间的总体及个体差异,为规范酶免检测提供实验数据。方法手工与全自动同时对同一组样本进行检测,运用统计学方法对获得的检测数据进行分析。结果手工检测变异系数为6.65%～21.38%,仪器检测变异系数为9.99%;结论各实验个体之间存在较明显差异,手工操作需要规范。
[Objective] To understand the general and individual difference between manual ELISA and automatic ELISA in the detection of HBsAg and provide laboratory data for the standardization of ELISA detection. [Methods] The samples were detected simultaneously by both manual and automatic ELISA; statistical method was used to analyze the acquired detection data.[Results] The variation coefficient of the manual detection was between 6.65% and 21.38%, while that of the automatic detection was 9.99%. [Conclusion] Significant difference exists between individuals; manual operation should be standardized.
出处
《职业与健康》
CAS
2009年第1期29-31,共3页
Occupation and Health
