硝苯地平性牙龈增生组织中成纤维细胞增殖和胶原分泌

Effects of cell proliferation and collagenous protein synthesis in Nifedipine responders

目的探讨硝苯地平对牙龈组织中成纤维细胞增殖和胶原分泌的影响。方法体外原代培养硝苯地平易感性牙龈组织和非易感性组织中人牙龈成纤维细胞。MTT法观察硝苯地平对细胞增殖的影响,酶联免疫吸附试验检测硝苯地平对细胞Ⅰ型胶原表达水平的影响。结果在第1,3,5天,硝苯地平易感性牙龈组织和非易感性组织之间Ⅰ型胶原合成和细胞增殖在统计学上均有显著性差异。结论细胞增殖明显和胶原合成增加可能和硝苯地平药物性牙龈增生机制有关。

Objective To investigate the effects of cell proliferation and collagenous protein synthesis in Nifedipine responders and non-respondee. Methods Gingival fibroblasts were primarily cultured from Nifedipine responders and non-responders in the presence of 1 μmol/L Nifedipine. The effects of cell proliferation were observed through evaluating MTT value. Enzyme-linked immunosorbent assay was taken to evaluate the amount of type I collagen. Results The expressions of collagen and cell proliferation were significantly different among Nifedipine responders and non-responders. Conclusion The expressions of collagen and cell proliferation may be associated with the biological mechanism for gingival overgrowth.