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Taqman探针检测VEGF基因多态性的实验研究 被引量：4

Experimental Factors Influencing Taqman Probe Allelic Discrimination in the Site of VEGFgene C-634G Polymorphism
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摘要 目的确定Taqman探针等位基因鉴别技术检测VEGF基因C-634G多态性的最适实验条件.方法对影响VEGF基因C-634G多态性Taqman探针检测的反应体系及探针、引物浓度等主要因素进行了系统研究.结果最适反应体系为12.5μL,最适探针、引物浓度为:0.3/12.5μL.结论建立了Taqman探针检测VEGF基因多态性的稳定实验方法. Objective To confirm the optimal test condition of the Taqman probe allelic discrimination in the Site of VEGF （vascular endothelial growth factor, VEGF） gene C-634G Polymorphism. Methods The factors influencing Taqman probe allelic discrimination in the site of VEGF gene C-634G Polymorphism were studied. Result The results revealed the optimum conditions：The effective reaction system was 12.5 μL; Taqman probe concentration was 0.3/12.5 μL. Conclusion The method for Taqman probe allelic discrimination in the Site of VEGFgene C-634G Polymorphism has been established stably.
作者 杨莹 张瑛 李晓进 李显丽 李惠民 陶文玉
机构地区 昆明医学院第四附属医院内分泌科 昆明医学院第四附属医院血液科
出处 《昆明医学院学报》 2008年第6期54-57,共4页 Journal of Kunming Medical College
基金 云南省教育厅基金资助项目(2007Z40625) 云南省第二人民医院基金资助项目(2008yk009)
关键词 TAQMAN探针 多态性 VEGF基因 实验条件 Taqman probe Polymorphism VEGF gene Test condition
分类号 R734.2 [医药卫生—肿瘤]
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参考文献5

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二级参考文献5

同被引文献26

  • 1张薇,单可人,吴昌学,李毅,肖雁,赵艳,齐晓岚,谢渊,何燕,官志忠,任锡麟.TaqMan-MGB探针检测GSTP1外显子5 SNP可行性研究[J].现代生物医学进展,2006,6(12):24-26. 被引量：2
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  • 8Awata T,Kurihara S,Takata N,et a1.Functional VEGF C(-634)G polymorphism is associated with development of diabetic macular edema and correlated with macular thickness in type 2 diabetes.Biochem Biophys Res Commun,2005,333:679-685.
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昆明医学院学报
2008年 第6期

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