两检测系统测定二氧化碳结合力的结果一致性分析

Evaluation of Consistency of CO_2-CP Testing Results between Two Biochemical Analytical Systems

目的评价我室两个生化检测系统间二氧化碳结合力CO2-CP测定结果的一致性.方法根据美国CLSI GP29-A文件进行样本分割实验,收集病人血清后一分为二,分别校准我室目前用于测定二氧化碳结合力的两个检测系统,并在该分析批内确认室内质控在控,在两检测系统分别进行双份测定,最后计算两系统之内的允许差异值,判断两检测系统是否能得出一致性的结果.结果两检测系统在8.7～45.6 mmol/L范围内测定二氧化碳结合力的相关性良好,其结果一致性可以接受.结论对于目前暂时没有室间质评计划的项目,运用美国CLSI GP29-A文件进行样本分割实验评价结果一致性是一种方便、快捷的手段.

Objective To evaluate consistency of CO2-CP testing results between two biochemical analytical systems. Methods Each biochemical analytical system was separately calibrated and ensured the daily IQC under control limit. According to CLSI document GP29-A, several patient serums were collected for experiment, every serum was divided into two and made duplicate CO2-CP testing on each biochemical analytical system, finally the allowable difference was calculated to evaluate results consistency. Results Two biochemical analytical systems had well relativity of CO2-CP testing from 8.7 mmol/L to 45.6 mmol/L concentration range, results consistency was accepted. Conclusion Split-sample testing is a convenient and fast alternative procedure to evaluateresults consistency for those items which proficiency testing is not available.