氟离子注入纯Ti表面改性对成骨细胞Ⅰ型胶原形成和表达的影响

INFLUENCE OF SURFACE MODIFICATION OF Ti BY FLUORINE ION-IMPLANTATION ON FORMATION AND EXPRESSION OF COLLAGEN-Ⅰ ON OSTEOBLAST

应用等离子体浸没离子注入装置对纯Ti表面进行氟离子注入,通过SEM和XPS分析研究材料的表面形貌和化学组成.将MG-63成骨细胞接种于氟离子注入前、后的Ti样品表面,采用激光共聚焦显微镜观察材料表面对成骨样细胞MG-63Ⅰ型胶原形成的影响;逆转录聚合酶链反应检测Ⅰ型胶原mRNA的表达;蛋白印迹法检测Ⅰ型胶原蛋白的表达.结果表明,氟离子注入后Ti样品表面新出现TiF_3;且MG-63细胞在其表面分别培养6,24和48 h后,Ⅰ型胶原的形成和表达均明显高于纯Ti表面.含氟表面改性层可提高Ti材料的生物相容性.

Commercial pure Ti discs were fluorine ion-implanted by plasma immersion ion implantation technique, and the morphology and chemical composition of the modified surface layers were characterized by SEM and XPS, respectively. MG-63 cells were seeded on the surfaces of modified Ti discs in 24-well cell culture plates at a density of 2 × 10^4 mL^-1 and examined by the molecular biology technique for 6, 24 and 48 h. It was confirmed by immunofluorescent staining that the formation of collagen-Ⅰ on the implanted Ti was significantly more than that on the pure Ti. Furthermore, the reverse transcription polymerase chain reaction and Western blotting showed that the expressions of mRNA and protein of collagen-Ⅰ on the implanted Ti were fairly greater than those on the pure Ti. It can be concluded that the modified surface containing fluorine is beneficial to improve the biocomoatibilitv of Ti.