非小细胞肺癌化疗敏感性与其临床生物学行为

The Study about Chemosensitivity of Non-Small Cell Lung Cancer and its Clinic Biological Behaviors

〔目的〕研究非小细胞肺癌对化疗药物原发敏感情况及肺部临床生物学行为对药敏的影响。〔方法〕采用MTT比色法测定31例非小细胞肺癌标本对9种化疗药物的敏感性，每种药物设3个浓度等级，根据每种药物的抑制率进行单因素方差分析。（结果）31例肺癌标本对9种化疗药物的敏感程度依次为；异长春花碱83.87％、长春新碱77.42％、丝裂霉素74.19％、阿霉素69.72％、卡铂69、72％、顺铂61．29％、足叶乙甙58．06%、氨甲喋呤22.58％、5氟脲嘧啶19．35％；随着9种化疗药物浓度的增加，其药物抑制率亦明显增加；足叶动式和阿霉素对鳞癌抑制率明显高于腺癌；足叶乙甙和顺铂对低分化肺癌抑制单明显高于中高分化肺癌。〔结论〕非小细胞肺癌作外药敏性受多个试验因素影响，并与非小细胞肺癌的病理类型和分化程度相关。

(Purpose) To research intrinsic sensitivity of non-small cell lung cancer (NSCLC) to chemotherapy and affection factors of clinic biological behaviors of NSCLC. (Methods) We detected 31 NSCLC samples by MTT colorimetric assay about chemosensitivity to 9 anticartinogens. Each drug had 3 dose dilutions. The result was analysed by the inhibition rate of each drug with one-way ANON.(Results) The chemosensitivity of 31 lung cancer Sampes was as below: Vinorelbine bitartate 83. 87%, Vincristine 77- 42%, Mitomycin 74. 19%, Adriamycin 69. 72%, Carboplatin 69. 72%, Cisplatin 61. 29%, Etoposide 58. 06%, Methotrexate 22. 58%, 5-Fluorouracil 19. 35%. The inhibition rate was abolutely increasing with dilution increasng of 9 anticaednogensi In squamous carinoma the inhibition rate of Etoposide and Adriamycin was obviously moe than that in adenocarinoma. In poor djfferntiation NSCLC the inhihition rate of Etopeide and Cisplatin was obviously more than that in moderate and high differentation NSCLC. (Conclusions) In vitro chemosenaitivity assay was affected by multiple tesl lactors. There were correlative between chemosensitivity of NSCLC and its degree of histopathologial and differentiation.