真皮多能干细胞与聚（对苯二甲酸丁二醇酯-co-聚乙二醇-co-低聚乳酸）三元共聚酯的生物相容性

Biocompatibility of PBTEOLA copolymers with skin-derived precursor cells

目的观察小鼠真皮多能干细胞（SKP）与聚（对苯二甲酸丁二醇酯-co-聚乙二醇-co-低聚乳酸）（PBTEOLA）三元共聚酯的生物相容性。方法分离培养小鼠乳鼠SKP。用DAPI标记SKP细胞核后，荧光显微镜下动态观察SKP接种在共聚酯支架上的生长情况；SKP与共聚酯体外立体培养至第4天和第7天时，通过扫描电镜及苏木素-伊红（HE）染色观察细胞生长及基质分泌情况；噻唑蓝（MTT）比色法检测共聚酯对SKP的增殖影响。结果SKP细胞接种于共聚酯上24h后即贴壁生长，第5～7天时为生长高峰。SKP牢固地黏附于共聚酯材料表面和孔隙内，细胞间有伪足相连，且有基质形成。检验时间因素和材料因素交互作用时，差异无统计学意义（P〉0．05），共聚酯对细胞生长无明显影响。结论PBTEOLA三元共聚酯对SKP细胞具有良好的生物相容性，可选择作为组织工程的支架材料。

Objective To explore the biocompatibility of poly（ 1,4-butylene terephthalate-co-eth- ylene oxide-co-DL-lactide） （PBTEOLA） copolymers with skin-derived precursor cells and investigate the possibility of PBTEOLA copolymers being used as scaffold in tissue engineering. Methods Skin-derived precursor cells were separated and cultured with PBTEOLA copolymers in vitro. Cells were examined by in- verted microscope, scanning electron microscope （SEM）, HE staining and staining of DAPI to assess cell growth and morphology. Cell adhesion and proliferation were detected by MTT assay. Results The SKPs grew adherently 24 h after plantation on the surface of PBTEOLA copolymer, and reached a growth peak after 5 to 7 days. HE stain and SEM scanning revealed that SKPs adhered on the surface and pore of copolymer, and linked with pseudopods and excreted cellular matrix. MTT showed that copolymer had no significantly negative effect on the growth of SKPs （ P 〉 0.05 ）. Growth curves of the cells in the experimental and control groups were alike and there was no statistically significant difference （P 〉 0.05 ）. Conclusion PBTEOLA copolymer has a good bioeompatibility with skin-derived precursor cells and has a potential to be used as scaffold in tissue engineering.