吸入一氧化氮对烟雾吸入性损伤犬肺组织含水量的影响

The effect of inhaled nitric oxide on pulmonary tissue water content in dogs after smoke inhalation injury

目的：观察吸入一氧化氮（ＮＯ）对烟雾吸入性损伤犬肺组织含水量的影响。方法：２１只犬随机分为３组：烟雾吸入后单纯吸氧〔氧浓度（ＦｉＯ２）０．４５〕为对照组（８只）；吸氧（ＦｉＯ２０．４５）＋０．００４５％ＮＯ为治疗组（９只），按时相点采血标本；正常组（４只）不致伤，用于建立组织学对照。动脉血浆胶体渗透压（ＣＯＰ）行多个样本均数间方差分析；支气管肺泡灌洗液（ＢＡＬＦ）中ＣＯＰ和蛋白质含量行两样本均数ｔ检验。结果：吸入ＮＯ治疗组血浆ＣＯＰ比对照组升高（Ｐ＜０．０５）；ＢＡＬＦ中ＣＯＰ比对照组略降低（Ｐ＞０．０５），而ＢＡＬＦ中蛋白质含量比对照组明显降低（Ｐ＜０．０５）；肺组织含水量略低于对照组（Ｐ＞０．０５）。结论：吸入ＮＯ对烟雾吸入性损伤犬肺组织含水量有减轻趋势，但尚无显著的效果，对其病理转归的影响仍难以定论，有待进一步深入研究。

Objective:To investigate the effect of inhaled nitric oxide (NO) on pulmonary edema in dogs after smoke inhalation injury.Methods:Twentyone dogs were randomly divided into 3 groups.Following smoke exposure,in the control group (n=8) O2 (FiO2 045) was inhaled,and in the treatment group(n=9) O2 (FiO2 045) and 00045 % NO were inhaled.In addition,4 dogs without smoke inhalation served as normal subjects.Plasma colloid osmotic pressure (COP) was analyzed using ANOVA for multiple mean comparisons,and contents of total protein and COP in brochoalveolar lavage fluid (BALF) were evaluated using the Student't test between two mean comparisons.Results:After inhalation of NO,plasma COP levels were significantly increased compared with the control group (P<005),without marked difference in COP levels in BALF between the 2 groups (P>005).However,total protein contents in BALF in the treatment group were much lower than that in the controls (P<005).No significant differences in pulmonary tissue water content were observed between the 2 groups (P>005).Conclusions:Following inhalation of NO,there is a tendency to mitigation of pulmonary edema induced by smoked inhalation injury in dogs,without other remarkable changes.Further studies are required to test the therapeutic advantage of this agent on pathologic alterations and outcome following inhalation injury.