亚硒酸钠和维甲酸对MNNG诱导人全血白细胞非程序DNA合成的影响

EFFECTS OF SODIUM SELENITE AND RETINOIC ACID ON MNNG-INDUCED UNSCHEDULED DNA SYNTHESIS OF HUMAN WHOLEBLOOD CELLS

本文应用非程序ＤＮＡ合成（ＵＤＳ）试验，观察了亚硒酸钠（Ｎａ２ＳｅＯ３）和维甲酸（ＲＡ）单独和联合作用对致癌物ＭＮＮＧ诱发的人全血白细胞ＤＮＡ损伤的影响。结果表明，Ｎａ２ＳｅＯ３和ＲＡ均具有抗诱变作用；当Ｎａ２ＳｅＯ３（０．１μｇ／ｍｌ）和ＲＡ（０．１μｇ／ｍｌ）联合作用时，也可明显降低ＭＮＮＧ诱发的ＵＤＳ值，其联合抑制作用大于Ｎａ２ＳｅＯ３的单独作用。实验结果还显示，Ｎａ２ＳｅＯ３作为抗癌剂，它在高剂量时又呈现遗传毒性。

The effects of sodium selenite(Na2SeO3) retinoic acid(RA) and Na2SeO3+RA were examined on MNNG-induced DNA damaged with human wholeblood cells by the test of unscheduled DNA synthesis (UDS) in vitro.The results showed that 0.01～0.05μg/ml Na2SeO3 and 0.02～2.0μg/ml RA significantly suppressed the responses of UDS induced by MNNG.These revealed that Na2SeO3 and RA had the antimutagenic activity.The combined inhibiting effect of (0.1μg/ml) Na2SeO3+(0.1μg/ml) RA on MNNG-induced DNA damage was stronger than the simply effect of Na2SeO3. The results also revealed that Na2SeO3,as inhibitor of mutagenesis,could induce genetoxic effects by itself at higher dose levels.