多核细胞检测法的方法学探讨

THE METHODOLOGY ON THE MULTINUCLEATED CELL ASSAY

本文应用Ｌ９（３）４正交试验设计对影响多核细胞检测法的三个主要因素进行了探讨。结果表明：６－ＴＧ终浓度为２×１０－５Ｍ，Ｃｙｔ－Ｂ终浓度为６ｍｇ／ｌ，７２小时收获细胞的组合为最佳实验搭配方案。同时对其它影响因素也作了研究。

Three main factors influcing the multinucleated cell assay were evaluated with the L 9(3) 4 orthogonal design. The result was the best when the 6-TG final density was 2×10 -5 M, the Cyt-B final density was 6mg/l, and the culture time was 72 hours. Meanwhile the other factors were also studied.