基于纳米自组装技术的功能化溶胶-凝胶过氧化氢生物传感器

H_2O_2 biosensor based on functional sol-gel network and nanoparticle self-assemble techniques

结合功能化溶胶-凝胶(sol-gel)网络结构、自组装技术和纳米粒子效应,提出一种生物传感界面构建方法。利用自组装技术在玻碳电极表面组装氨基化sol-gel膜,通过与自组装膜间的强烈作用将纳米金粒子固定于sol-gel网络中,再通过静电吸附作用实现辣根过氧化物酶(HRP)在纳米金粒子表面的固定化,构建纳米自组装HRP传感界面。将制备的传感器用于对H_2O_2的催化还原,很好地保持了酶的生物活性,改善了传感器的灵敏度。

A novel immobilization method of horseradish peroxidase applied to a biosensor for detecting hydrogen peroxide has been proposed by incorporating functional sol-gel fill and nanoparticle serf-assemble techniques. The interfacial design of biosensor was prepared by serf-assembling the amino-containing sol-gel onto the glass carbon electrode which surface present high concentration of reactive hydroxyl/oxide groups, then chemisorbing gold nanopartieles into the amino-containing sol-gel networks, and finally electrostatically adsorbing horseradish peroxidase onto the surface of the gold nanoparticles. Cyclic voltammetry was employed to investigate the electrochemical characteristics of the biosensor in 5 mmol/L K3Fe（CN）6 -0.1 mol/L KNO3 -0.05 mol/L PBS system. The conditions of the biosensor were evaluated with respect to the serf-assembly time, the size of the gold nanoparticles, sensitivity as well as stability. Satisfactory results were obtained when this biosensor was applied to catalytic reduction of hydrogen peroxide.