血涂片血小板计数方法的意义

Clinical value of platelet count with blood smear method

目的探讨血涂片血小板计数方法的意义。方法取抗凝静脉血制成血涂片,经瑞氏染色后普通光学显微镜下间接计数血小板,将其结果与血细胞分析仪上测出血小板计数和手工法计数进行比较。结果配对t检验比较,血小板降低组血涂片法与血细胞分析仪计数进行比较,差异有统计学意义(P<0.01),与手工法计数血小板结果相比较,差异无统计学意义(P>0.05),血小板正常组和增高组采用血涂片法分别与血细胞分析仪计数和手工法计数进行比较,各方法间差异无统计学意义(P>0.05)。结论结果显示,对血小板正常或升高的标本,血细胞分析仪计数结果比较准确,但对血小板降低的标本,计数结果准确性较差。血涂片法间接计数血小板直观、准确,具有一定的临床应用价值,对血细胞分析仪有复核作用。

Objective To discuss the clinical value of platelet count with blood smear method. Methods Anticoagulant venous blood was collected and prepared as blood smears,then treated with Wright＇s staining and observed under ordinary optical microscope for indirect counting of platelets. The results were compared with the measured values via hematocyte analyzer and manual method. Results Paired T test showed there was statistical difference in platelet count between blood smear method and hematocyte analyzer counting （P〈0.01） in plate-reduction group, but no statistical difference was found between blood smear method and manual method （P〈0.05）. Nevertheless, there was statistical difference in platelet count between blood smear method and hematocyte analyzer counting as well as manual method in platelet normal group and elevation group （P〈0.05）. Conclusion The results show that the hematocyte analyzer counting is accurate for samples with normal or elevated platelet count, whereas inaccurate for samples with reduced platelet count. Blood smear method is direct and accurate, having a certain clinical value and contributing to recheck of measured results with the hematocyte analyzer.