摘要
目的:观察抗癌中药"卡宁"对体外培养的肝癌细胞株BEL-7402细胞的凋亡抑制基因bcl-2的影响,探讨其抗肿瘤治疗的作用机制。方法:取体外培养的对数生长期BEL-7402细胞,随机分为4组:对照组,5-氟尿嘧啶组,含药"卡宁"血清1组(10%含药血清组)和含药"卡宁"血清2组(15%含药血清组);采用MTT比色法观察不同浓度含药血清对体外培养的人肝癌细胞株BEL-7402细胞的抑制能力,PCR检测其对凋亡抑制基因bcl-2的影响。结果:PCR结果显示,"卡宁"1、2组bcl-2的活性明显低于对照组(P=0.038);MTT结果显示,细胞生长抑制率随含药血清作用时间及浓度的增加而增大(P<0.05),15%含药血清72h的作用最强。结论:对肿瘤细胞bcl-2的抑制可能是抗癌中药"卡宁"诱导肿瘤细胞凋亡作用机制的一部分。
Objective： To observe the effects of anticancer chinese medicine ＂Caning＂ on the anti-apoptosis gene bcl-2 of in vitro cultured human hepatic cancer cell line BEL-7402 cells, and investigates its anticancer therapeutic mechanism. Methods： In vitro cultured BEL-7402 cells in a logarithmic growth phase were randomly divided into 4 groups： control group, 5-Fluorouracil treated group, serum group 1 containing ＂Caning＂ （10% pastille serum） and serum group 2 containing ＂Caning＂（15% pastille serum）; then MTT assay was employed to detect the inhibitory ability of serums containing different concentrations of ＂Caning＂ to in vitro cultured human hepatic cancer cell line BEL-7402 cells, and PCR was used to detect the effect of ＂Caning＂ on the anti-apoptosis gene bcl-2. Results： Results of in vitro experiment suggested that, the bcl-2 gene activity in serum group 1 and 2 containing ＂Caning＂ was significantly lower than that of control group （P=0.038）; result of MTT assay indicated that the cell growth inhibitory rate was increased along with the increase of the action time and concentration of serum containing ＂Caning＂（P〈0.05）, and the 15% pastille serum showed the strongest inhibitory effect at 72 h. Conclusion： ＂Caning＂ could significantly inhibit the growth of hepatic cancer cells partly by inhibiting the anti- apoptosis gene bcl-2.
出处
《中国医药导报》
CAS
2009年第1期14-16,共3页
China Medical Herald
基金
广东省中医药管理局科技项目(编号:1040105)
