黔产木犀科苦丁茶及加工品中总黄酮含量测定

Determination of total flavonoids in Kudingcha of Oleaceae from Guizhou Province and its processing products

目的:测定黔产木犀科苦丁茶及加工品的总黄酮含量,为正确评价其质量提供一定的科学依据。方法:采用分光光度法,以芦丁为对照品,在507nm处测定吸收度。结果:总黄酮最高含量为39.71mg/g,最低为12.11mg/g;产品中嫩叶的总黄酮含量比老叶中的含量要低。结论:该法操作快速简便,具有良好的稳定性、准确性和重现性,可用于苦丁茶药材及其加工品的测定。

Objective： To determine the content of total flavonoids in Kudingcha of Oleaceae from Guizhou Province and its processing products. Methods： The total flavonoid was determined by UV spectrophotometry at 507 nm, with rutin as a reference substance. Results： The content of flavonoids had different scales in Kudingcha of Oleaceae from Guizhou Province and its processing products. The highest contents were 39.71 mg/g and the lowest were 12.11 mg/g. The total flavonoids in tender leaves of processing products were lower than that in old leaves. Conclusion： The method is simple, accurate and reliable, and it can be used for the determination of the total flavonoids in Kudingcha of Oleaceae from Guizhou Province and its processing products.