白藜芦醇对大鼠脑缺血再灌注损伤抗炎机制研究

Study on anti-inflammatory mechanisms of resveratrol on cerebral ischemia reperfusion injury of rats

目的:研究白藜芦醇对大鼠局灶性脑缺血再灌注损伤抗炎作用机制。方法:大鼠脑缺血再灌注模型完成后,缺血2h再灌注24h,其中白藜芦醇大剂量组(40mg/kg)、小剂量组(10mg/kg)术前7d分别腹腔注射2ml/kg白藜芦醇无菌溶液,模型组腹腔注射2ml/kg生理盐水,1次/d,各组大鼠均于手术前15min加注1次。观察不同剂量的白藜芦醇对脑组织一氧化氮(NO)含量、髓过氧化物酶(MPO)活性和脑组织神经细胞的细胞间黏附分子-1(ICAM-1)蛋白的表达的影响。结果:白藜芦醇可显著减轻大鼠脑缺血再灌注损伤的行为评分,显著降低脑组织中NO含量和MPO的活性,差异有统计学意义(P<0.05);ICAM-1蛋白的阳性表达率降低,差异有统计学意义(P<0.05)。结论:白藜芦醇对大鼠脑缺血再灌注损伤有保护作用,作用机制之一可能与其抑制炎性反应有关。

Objective： To investigate the anti-inflammatory mechanisms of resveratrol on cerebral ischemia reperfusion injury （CIRI） of rats. Methods： The model of MCAO was established by intraluminal suture technique. After 2 h of occlusion and 24 h of reperfusion, the rats of the high dose resveratral （40 mg/kg） group and low dose resveratrol （10 mg/kg） group were injected by intraperitoneal injection for 7 days, respectively, and the control group were given normal saline（2 ml/kg）, one time a day, and all groups were given a more time in 15 minutes before operation. The contents of NO and activities of MPO in brain tissue of ischemic hemisphere were measured; the expression of ICAM-1 in the cortex were measured by immunohistochemical method. Results： The neurological scores of rats treated with resveratrol was reduced apparently compared with model group rats （P〈0.05）; there were apparent reduction of the contents of NO and marked decrease of MPO in Resveratrol group compared with the I/R model group （P〈0.05）; compared with I/R model group, the expression of ICAM-1 was down-regulated obviously in resveratral group （P〈0.05）. Conclusion： Resveratrol could relieve the cerebral ischemia reperfusion injury, one of the mechanisms could be the anti-inflammatory effect of procyanidin.