银杏达莫注射液与川芎嗪对实验糖尿病肾病大鼠NO的作用

Effect of Ginkgo Leaf Extract and Dipyridamole Injection and Ligustrazine on Nitric Oxide in Experimental Diabetic Nephropathy in Rats

目的:研究银杏达莫注射液(杏丁)、川芎嗪对实验糖尿病大鼠肾脏NOS系统的作用。方法:以链脲佐菌素(STZ)制备动物模型。实验大鼠分为四组:糖尿病对照组A(16只,分早、晚期对照各8只),杏丁治疗糖尿病肾病组B(16只,分早、晚期对照各8只),川芎嗪治疗糖尿病肾病组C(16只,分早、晚期对照各8只),正常组D(16只,分早、晚期对照各8只)。分别测定第1周末尿微白蛋白(UAE)、肌酐清除率(Ccr),各期尿及肾皮质NO_2^-/NO_3^-、肾皮质NOS活性、肾脏病理分析。结果:①第1周末肌酐清除率(Ccr)、尿微白蛋白(UAE)在A、B、C组均明显升高;②A组早期尿及肾皮质NO_2^-/NO_3^-、肾皮质NOS活性明显升高,晚期明显下降;③B组、C组杏丁、川芎嗪治疗后均能使尿及肾皮质NO_2^-/NO_3^-、肾皮质NOS活性升高;④与A组比较B、C组早期治疗肾小球FN含量明显增加,B、C组晚期治疗则明显下降。结论:①糖尿病肾病早期NO增加,随之下降。②杏丁、川芎嗪能作用于糖尿病患者的NOS系统,使NO增加。杏丁、川芎嗪使NO增加的结果导致早期糖尿病肾脏的损害加重,晚期则有保护肾功能的作用。实验结果为临床正确使用杏丁、川芎嗪提供了理论依据。

To investigate that the effective of Ginkgo Leaf extract and Ligustrazine on nitric oxide in experimental diabetic nephropathy in rats. Method： Rats were treated with 60mg-kg^-1 of stretozotocin （STZ） to mimic diabetes model and randomly divided into four groups ： diabetes rats （ group A, n = 16）, diabetes nephropathy rats ＋ Ginkgo Leaf extract （ group B, n = 16 ）, diabetes nephropathy rats ＋ Ligustrazine（ group C, n = 16） and normal control rats （group D, n = 16）. The urinary microalbumin excretion （UAE）, Ccr, urinary and renal cortex NO2^-/NO3^- , NOS in renal cortex were measured and morphlogical was observated. Result：①Ccr and UAE were increased significantly in group A , group B and group C compared to group D after 1 week. ②urinary and renal cortex NO2^-/NO3^- , NOS were significantly increased in group A and B compared to group D expect group B in 2 weeks. ③Ginkgo Leaf extract and Ligustrazine can inprove NO2^-/NO3^- , NOS in group B and C compared to group A. ④FN was increased in group B and C of early stage and decreased in group B and C of advanced stage compared to group A in 8 weeks. Conclusion： ①Nitric oxide was initially increased and then gradually decreased in the development of diabetic nephropathy. ②Ginkgo Leaf extract or Ligustrazine can act on NOS system of diabetes patients, increasing NO synthesis, which led to worsen renal injury in early stage of diabetes nephropathy, however, it had protective effect on the renal in the late stage. These results provided that how to use Ginkgo Leaf extract or Ligustrazine correctly in the clinic.