复合维生素B泛酸钙口服溶液中维生素B_1和维生素B_6的含量测定

Simultaneous Determination the Contents of Vitamin B_1 and Vitamin B_6 in Compound Vitamin B-calcium Pantothenate Oral Solution

目的:建立HPLC法同时测定复合维生素B泛酸钙口服溶液中维生素B_1和维生素B_6的含量。方法:采用Elite C_(18)色谱柱(250mm×4.6mm,5μm),流动相为乙腈-庚烷磺酸钠溶液(10:90);检测波长为280nm;流速:1.0ml·min^(-1)。结果:维生素B_1及维生素B_6的线性范围分别为19.8～198.4μg·ml^(-1)(r=0.999 9)和6.2～62μg·ml^(-1)(r=0.999 9)。维生素B_1平均回收率为99.8%,RSD为0.4%;维生素B_6平均回收率为99.9%,RSD为0.6%(n=6)。结论:方法快速、简单、准确,可作为该制剂的质量控制方法。

To establish an HPLC method for simultaneous determination the contents of Vitamin B1 and Vitamin B6 in compound vitamin B-calcium pantothenate oral solution. Method： The Elite C18 column （250mm × 4.6mm,5μm）was used, the acetonitrile-sodinm heptane sulfonate solution （10：90） as mobile phase, the flow rate was 1.0 ml·min^-1, the detection wavelength was 280nm. Result： The linear ranges of VB1 and VB6 were at 19.8 - 198μg·ml - 1 （ r = 0.999 9 ） and 6.2 - 62μg·ml - 1 （ r = 0. 999 9 ） , the average recoveries （n = 5 ） were 99.8% （RSD = 0.4% ） and 99.9% （RSD = 0.6% ） respectively. Conclusion： The method is simple, accurate, and reliable, it can be used for quality control of the oral solution.