摘要
目的:建立高效液相色谱法测定对羟基苯甲酸甲酯的含量及有关物质。方法:采用C_(18)色谱柱(150 mm×4.6 nm,5μm);以甲醇-水-冰醋酸(50:50:0.1)为流动相;流速1.0 ml·min^(-1);检测波长256 nm。结果:对羟基苯甲酸甲酯在5.2～208μg·ml^(-1)线性范围内,线性关系良好(r=0.999 9),最低检测限为0.03ng。结论:本法简便、准确、重复性好,可用于对羟基苯甲酸甲酯的含量测定和有关物质检查。
To establish an HPLC method for the determination the contents of methylparaben and related substances. Method： C18 column （150 mm ×4.6 nm,5μm） was used, the mobile phase was composed of methanol-water-acetic acid glacial （50 ： 50：0.1 ） , the detection wavelength was 256 nm. Result： The calibration curve was linear in the range of 5.2 - 208μg·ml^-1（ r = 0. 999 9 ）. The detection limit was 0.03ng. Conclusion： The method is simple, accurate and repeatable which can be applied to determination the contents of methylparaben and related substances.
出处
《中国药师》
CAS
2009年第1期70-72,共3页
China Pharmacist
