摘要
目的:建立用HPLC法测定复方玄驹胶囊中淫羊藿苷含量的方法。方法:采用Agilent Zoxbax·XDB-C_(18)柱(150mm×4.6mm,5μm);流动相:乙腈:水:磷酸=25:75:0.5;流速:1.0ml·min^(-1);检测波长:270nm。结果:淫羊藿苷进样量在0.07～1.12μg范围内具有良好的线形关系(r=0.999 7),加样回收率为99.27%,RSD为0.81%。结论:本方法灵敏、简便、快速,适合于该药的质量控制。
To establish the HPLC method for determining the content of icariin in Fufangxuanju capsule. Method： Separation was achieved on a ZORBAX XDB-C18 column（ （4.6mm × 150mm,5μm）with a ethanol-water-phosphoric acid solution（25： 75： 0.5） as mobile phase and detection wavelength at 270nm. The flow rate was 1.0ml ·min^-1. Result： There was a good linear relationship whin the range of 0.07μg - 1.12μg （ r = 0. 999 7 ）. The recovery was 99.27 % with RSD 0.81%. Conclusion： The method is simple, rapid and accurate for qualitv control of Fufangxuanju capsule.
出处
《中国药师》
CAS
2009年第1期81-82,共2页
China Pharmacist
