人结肠癌Caco-2细胞对自组装海藻酸钠纳米粒摄取的体外研究

Uptake of Self-assembled Sodium Alginate Nanoparticles in Human Colon Carcinoma Caco-2 Cell in Vitro

目的:研究人结肠癌Caco-2细胞对异硫氰基荧光素(FITC)标记的自组装海藻酸钠纳米粒(sSAN-FITC)的摄取及影响因素。方法:Caco-2细胞加入sSAN-FITC孵育后采用荧光显微镜检测细胞对sSAN-FITC的摄取情况;通过采用荧光酶标仪检测细胞内FITC的荧光强度,观察sSAN浓度、作用时间、环境pH值对细胞摄入sSAN的影响,并与空白对照组比较。结果:sSAN可以被Caco-2细胞摄入;与空白对照组比较,3种影响因素中,随着sSAN浓度升高及作用时间延长,荧光强度增强(P<0.01),当pH值低于或高于7.0时,荧光强度均增强(P<0.01或P<0.05)。结论:sSAN可被Caco-2细胞摄取,且呈浓度、时间依赖性,并受pH值的影响。

OBJECTIVE： To investigate the uptake of self assembled sodium alginate （SA） nanoparticles labeled by FITC （sSAN- FITC） in Caco-2 cells and the influencing factors. METHODS： Fluorescence microscope was utilized to exam the uptake of sSAN - FITC in Caeo - 2 cells. Multifunction continuous spectrum enzyme - linked imnmnosorbent assay system was used for the detection of the fluorescence intensity of FITC in the cells and quantitative assay of the effect of sSAN concentration, its action time and pH on the uptake of sSAN FITC in Caco- 2 ceils, with blank group as control. RESULTS： Pictures of Caco 2 cells taken by fluorescence microscope showed uptake of sSAN - FITC by Caco - 2 cells. As compared with blank control group, among the 3 factors, the fluorescence intensity increased with the increase of the sSAN concentration and the prolonging of the action time （P 〈 0.01） . And the fluorescence intensity increased in cells when pH was either higher or lower than 7.0 （P〈0.01 or P〈0.05） . CONCLUSIONS： Uptake of sSAN by Caco 2 cells was noted in a concentration and time dependent manner, and the uptake is also influenced by the pH of the culture solution.