摘要
目的建立柴黄颗粒中黄芩苷的含量测定方法。方法采用Alltima C18（5μm，150mm×4．6mm）色谱柱；流动相为甲醇-0．3％磷酸溶液（50：50）；检测波长为280nm。结果黄芩苷峰与其他杂质峰分离良好，在0．3302-0．7706雌范围内，黄芩苷线性关系良好（r=0．9998），平均回收率为101．05％（n=2）RSD=1．39％。结论该方法简便易行，准确度高，重现性好，可用于柴黄颗粒的质量控制。
Objective To establish a method to determination of the contents of Baicalin in Chaihuang Granules. Method Alltima C18 （5 μm, 150 mm ×4. 6 mm） was used with the mobile phase consisting of Methanol -0.3% HaPO4 （50：50 ）, the detection wavelength was at 280 nm. Result The linear range of Baiealin was in the range of 0. 3302 - 0. 7706 μg, r = 0. 9998, the average recovery as 101.05 %, RSD = 1.39% （ n =9）. Conclusion The method is simple,accurate and credible. It can be used for the quality control of Chaihuang Granules.
出处
《世界科技研究与发展》
CSCD
2008年第6期711-712,共2页
World Sci-Tech R&D
关键词
柴黄颗粒
黄芩苷
含量测定
Chaihnang Granules
Baiealin
determination
