摘要
采用RT-PCR方法从体外诱导的水貂外周血淋巴细胞中扩增出水貂的α-干扰素基因(MiIFN-α)。测序结果显示,水貂的IFN-α基因序列全长为564个核苷酸,共编码187个氨基酸,与GenBank上已发表的雪貂的IFN-α核苷酸序列同源性为97.5%,氨基酸同源性为92%。
Interferon-Alpha gene from mink was amplified by PCR using primers based on highly conserved amino acid sequence blocks of dog＇s alpha WN genes. The cloned IFN gene contained 564 nucleotides and was deduced 187 amino acids. These results confirmed that the homology of nucleotides and amino acids was 97.5% and 92% respectively with the previously published Momestic ferret IFN-α gene.
出处
《特产研究》
2008年第4期6-9,共4页
Special Wild Economic Animal and Plant Research
基金
吉林省科技发展计划项目(20060550)
