高效液相色谱法测定当归中阿魏酸的含量

Determination of Ferulic Acid in Radix Angelicae Sinensis by High Performance Liquid Chromatography Method

目的：建立一种高效液相色谱法测定当归中阿魏酸的含量．方法：采用高效液相色谱法，色谱柱为TIANHE C18（250mm×4．6mm，5μm），以甲醇-水-冰醋酸（40：60：0．5）为流动相，流速为1mL／min，检测波长为320nm．结果：阿魏酸在0．036—0．216μg范围内，线形关系良好（r=0．9999），平均回收率为101．4％（n=5），RSD为0．76％．结论：该方法测定阿魏酸含量简便、快捷、准确．

Objective：A high performance liquid chromatographic （HPLC） method has been developed for determination of ferulic acid in radix angelica sinensis. Method：The ferulic acid was determined by HPLC. A 250 mm× 4.6 mm, TIANHE C18 column with mobile phase MeOH ： H2O ： CH3 COOH （40 ： 60 ： 0.5）, at flow rate 1.00 mL·min^-1 and detection at wavelength of 320 nm. Result： The linear range of ferulic acid was in the ranges of 0. 036- 0. 216 ug, （r = 0. 9999）, and the recovery was 101.4 % with RSD 0.76 % （n = 5）. Conclusion ： HPLC was a simple, fast and accurate method for ferulie acid analysis.