青杄花粉保存方法试验研究

Experiment on Protecting Pollen of Picea wilsonii

通过对不同保存方式下保存2～4年的同株青杄花粉生活力测定，结果表明：室温保存的青杄花粉效果较差，2年后花粉活力只有11．5％；低温保存条件下，随着保存时间的延长，花粉的生活力虽有下降，但不太明显，在4℃下和-20℃下保存青杄2年后，其生活力仍然在78％和80％左右，-20℃下保存的青杄花粉生活力较4℃稍强。因此，在-20℃条件下较长时间保存云杉花粉效果更为理想。

According to measuring the vitality of pollen ofPicea wilsonii from the same tree protected for 2 -4 years under different methods, the results show that the vitality of pollen ofPicea wilsonii is only 11.5% after two years stored at room temperature. Under the condition stored at low temperature, the vitality of pollen decrease with the prolongation of protecting time. After protecting pollen of Picea wilsonii at 4℃ and -20℃ for two years, the vitali- ty of pollen can still keep 78% and 80%, with the vitality of pollen protected at -20℃ be stronger than that one at 4℃. So, to protect pollen ofPicea wilsonii at -20℃ for longer time can reach the ideal effect.