低剂量γ射线全身预照射对荷鼠肿瘤浸润性淋巴细胞的影响

Effects of Low Dose γ-Rays Irradiation on Yield of Tumor-Infiltrating Lymphocytes in Mice

不同剂量γ射线全身照射小鼠后3、6、9、24h接种文氏腹水癌细胞。种植后第10天用不连续密度梯度离心法分离TIL，计数共得率．结果，种植肿瘤细胞前小鼠受到5～50cGyγ射线照射，不同程度地提高了TIL得率，其中以种植前6h10cGy照射最具统计学意义（P＜0．01）。说明低剂量电离辐射能提高免疫淋巴细胞在肿瘤组织局部的浸润程度，可能与其抑瘤作用有关。

It is confirmed that low dose irradiation can inhibit tumor growth. In order to know tumor growth inhibiting mechanism, the changes of tumor -infiltration lymphocytes (TIL) were investigated after exposing to tumor - bring mice. The mice were exposed to different doses. then, EAC cells were transplanted at the 3,6,9 and 24th hour. Ten days later TILs were separated by discontenuous density gradient method and the cell number were counted. The yield of TIL increased obviously caused by of 5 -10 cGy γ - rays irradiation. The most obvious increasing occured in the group in which cells was exposed irradiation for 6 hours at 10 cGy dose. A low dose radiation can make the yield of TIU increased. It might be correlated to the mechanism of tumor grouth inhibiting.