胸腺注射供体脾细胞诱导移植心免疫耐受的实验研究

Intrathymic Injection of Donor Spleen Cells Induces Specific Tolerance to Cardiac Allografts

本实验采用大鼠腹部心脏移植模型，在移植前21天将供体脾细胞分别注射到受体的胸腺、脾脏、静脉、皮下，然后行异位心脏移植，术后1周每日腹腔注射环孢素A（CsA）10mg／kg。胸腺注射供体脾细胞联合应用CsA组移植心平均存活时间较其它组显著延长，受体对供体靶细胞没有出现细胞介导的细胞毒作用．结果表明：胸腺注射洪体脾细胞联合应用CsA能成功诱导免疫耐受，胸腺内特异性T细胞克隆消除可能与免疫耐受的形式有关．

The induction of donor - specific tolerance would eliminate the risk of long -term immunosuppression while ensuring allograft function and survival. Male SD rats were exposed to donor alloantigen by an intrathymic, intrasplenic, subeutaneous or intravenous injection of 2.5 ×107 male Wistar splenocytes, and 21 days later received a heterotopic Wistar heart transplant. In addition, cyclosporine A (CsA, 10mg/kg) was given for 7 days by peritoneal injection (i. p.) post - operahvely. Intrathymic injection of spleen cells combined with short course of CsA treatmenl could significantly prolong heart allograft. Our results confirmed the role of the thymus as a special site for the induction and maintenance of specific immunological unresponsiveness to organ allografts. T cell clonal deletion may play an important role in this immune tolerance.