军蛭煎剂对小鼠胸腺、脾脏重量指数及组织结构影响的实验研究

Experimental Study of Junzhi Jianji on Weight Indices of Immune Organs and Histological Structure of Spleen of Aging Mice

目的:探讨军蛭煎剂对小鼠胸腺、脾脏重量指数及脾脏组织结构的影响。方法:将昆明种6月龄小鼠60只随机分为正常对照组、模型组、VE胶丸组(20mg/kg)、军蛭煎剂大剂量组(56.8mg/kg)、军蛭煎剂中剂量组(28.4mg/kg)、军蛭煎剂小剂量组(14.2mg/kg)。模型组和给药组每天腹腔注射D-半乳糖125mg/kg,正常组注射等量生理盐水,连续注射5w。5w后取材,称重,制备光镜标本。用电子天平称胸腺、脾脏湿重,以每10g体重中胸腺或脾脏所占重量(mg/10g)表示胸腺、脾脏重量指数。结果:与模型组比较,军蛭煎剂各剂量组和VE胶丸组小鼠脾指数和胸腺指数增加,脾脏显微结构改善。结论:军蛭煎剂具有改善D-半乳糖致衰小鼠免疫器官结构的形态学损坏,提高免疫器官重量指数的作用。

Objective： To observe the effect of Junzhi Jianji on weight indices of thymus gland and spleen and histological structure of spleen of aging mice. Methods： 60 Kunming mice of 6 months were divided into 6 groups randomly： control group, model group, VE group, Junzhi Jianji group 1 （high dosage）, group 2 （middle dosage）, group 3 （low dosage） . Demencia model mice were made by intraperitoneally injecting D-glacatose once a day for successive 5 w, while the mice in control group were injected with the same amount of physiological saline. 5 w later, the weights of spleen and thymus gland of mice were measured, histological structure of spleen was observed by light microscope. Result： Compared with model group, weight indices of spleen and thymus gland were increased and morphology impairment of histological structure of spleen was improved in every treatment group. Conclusion： Junzhi Jianji could increase weight indices of inunune organs and improve morphology impairment of histological structure of spleen.