乙型肝炎病毒血清标志e抗原两种模式在不同病毒复制水平中的分布及其与丙氨酸转氨酶的关系

The Distributions of two HBeAg Models in Different Levels of Viral Replication and its Relationship with ALT

〔目的〕分析乙型肝炎病毒感染者HBV血清标志e抗原(HBeAg)的阳性与阴性模式在不同病毒复制水平中的分布,并分析这2种模式与丙氨酸转氨酶(ALT)的相关性。〔方法〕应用酶联免疫吸附试验(ELISA)检测HBV感染血清标志,运用荧光实时定量PCR法测定HBV的拷贝数,采用速率法测定血清内的ALT。〔结果〕随着病毒复制水平的升高,HBsAg和HBeAg阳性乙肝病毒感染者的检出率显著增加,HBsAg阳性和HBeAg阴性乙肝病毒感染者的检出率具有逐渐减少的趋势;而且HBsAg和HBeAg阳性组的ALT水平要显著高于HBsAg阳性和HBeAg阴性组(P<0.001)。〔结论〕HBeAg阳性检出率与病毒复制水平和ALT异常的检出率间存在相关性,进一步证实了HBeAg阳性是反映HBV复制水平的可靠指标,可作为疾病进展的参考依据。

Objective To analyze the distribution of HBeAg（＋） and HBeAg（-） groups in different levels of viral replication, its relationship with ALT, and these two models in individuals of positive HBsAg. Methods The serum makers, HBV DNA and ALT were detected by ELISA, real-time PCR and rate assay respectively. Results The trend of increase in the rate of HBsAg and HBeAg positive group was observed over the increasing in HBV replication level. Abnormal rate of ALT significantly increased in HBsAg and HBeAg positive group. Conclusion It suggested that HBsAg and HBeAg was referenced indicator of HBV replication level, and allowed as referenced proof of disease progress.