用RP-HPLC法测定无花果叶中补骨脂素的含量

Determination of content of psoralen in fig leaves by RP-HPLC method

目的：建立无花果叶中补骨脂素的含量测定方法，以服务于今后的资源开发。方法：采用RPHPLC法，色谱柱KromacilC18柱（150mm×4．6mm，5μm），流动相甲醇：水（58：32），流速0．8mL／min，检测波长246nm，进样量80μL。结果：在1．256～125．6 μg／mL范围内，补骨脂素浓度与峰面积呈良好线性关系（r＝0．9999）。同一采集时间、同一树枝的不同叶片中补骨脂素的含量存在明显差异。结论：本文所建立的方法简便，重现性好，可用于无花果叶中补骨脂素的含量测定；无花果叶中补骨脂素含量因叶而异。

Objective： To establish an assay method for psoralen in fig leaves for further resource development. Methods： RP-HPLC method was adopted with Kromacil C18 column （150 mm× 4.6 mm,5 μm）,methanol water （58 ： 32） as mobile phase at flow rate of 0.8 mL/min and 246 nm as detecting wavelength. Results： The linear range of psoralen was 1. 256-125.6 μg/mL（r=0. 999 9）. There was apparent difference in psoralen content between different leaves of the same branch at the same collecting time. Conclusion： The method, which is simple with good reproducibility, can be applied in the assay of psoralen in fig leaves. The content of psoralen varies in different fig leaves.