山羊痘病毒SYBR Green I荧光PCR检测方法的建立

Establishment of real-time PCR technique for goat pox virus detection using SYBR Green I

为建立山羊痘病毒(goat pox virus,GPV)的SYBR Green I荧光PCR方法,根据GPV的反向末端重复序列(Inverted Terminal Repeat,ITR)的核苷酸序列设计引物。以此引物和山羊痘皮肤痘疹提取的DNA为模板,建立并优化SYBR Green I模式荧光PCR检测GPV的ITR基因的方法,并与普通PCR方法进行敏感性比较。结果表明,此次建立的检测GPV的SYBR Green I荧光PCR方法能检测出7×102拷贝数的DNA模板,此法比文献报道的普通PCR法更敏感、准确、快速。

A pair of primers were designed for detection of Goat pox virus （GPV） ITR （ Inverted Terminal Repeat） gene by using SYBR Green I real - time PCR technique. The results showed that SYBR Green I real - time PCR technique could detect 7 ×10^2 copies of the DNA template, this assay was more sensitive than the conventional PCR assay reported previously.