猪圆环病毒2型的PCR快速检测及其临床应用

The rapid detection of porcine circovirus type 2 based on PCR and its clinical application

根据GenBank中发表的猪圆环病毒2型(porcine circovirus type 2,PCV2)基因组序列,设计了2段特异性核苷酸引物.通过对已知PCV2和对照病毒(PCV1、伪狂犬病毒、猪细小病毒)的PCR扩增,证明所设计的引物可用于PCV2的特异性PCR检测.采集来自江苏扬州和泰州地区的157头份临床门诊病料(每头份均为脾脏和腹股沟淋巴结双份病料)进行PCR检测,发现仅从腹股沟淋巴结检出PCV2的占21.65%,仅从脾脏检出PCV2的占7.64%,从脾脏和淋巴结均可检出PCV2的占41.40%,其中扬州市地区感染率为73.47%,泰州地区感染率为66.10%.通过对扬州某屠宰场的32份腹股沟淋巴结的检测,结果表明临床健康猪的PCV2隐性感染率也高达71.88%.

As porcine circovirus type 2 （PCV2） is an important pathogen in swine,a PCR method was established by using a set of primers derived from the published nueleotide sequence of the PCV2 genes deposited in GenBank. In order to confirm its specificity, the PCR was performed by using the template DNAs from the reference virus,and the expected products of 573 bp were only detected in PCV2,whereas no specific band was detected in PCV1 or other virus such as pseudorabies virus （PRV） and porcine parvovirus （PPV）. To further investigate the prevalence of PCV2, spleen and lymph node samples were obtained from 157 hoggeries in Yangzhou and Taizhou region from July to September 2006,and submitted to PCR detection. The data indicated that 111 hoggeries were in danger of PCV-2 infection, while Yangzhou is 73.47% and Taizhou is 66.10 %. Furthermore,32 inguinal glands from the slaughterhouse in Yangzhou were also tested, and the data revealed that the PCV2 silent infection rate in clinical healthy swine was 71.88 %.