镍离子诱导牛血清白蛋白构象变化的动力学机理研究

Mechanism for conformation transition of BSA induced by Ni ion

用差示紫外吸收光谱法研究了镍离子与牛血清白蛋白(BSA)相互作用的动力学过程,发现它们之间的相互作用是一个连串反应过程,首先是镍离子与BSA的一个快速络合反应,然后是镍离子诱导BSA发生了构象变化,这个构象变化过程是一个正向和逆向均为一级反应的对峙反应,而不是文献报道的简单一级反应。

The kinetics of conformational transition in the interaction of Ni ion with bovine serum albumin （BSA） was studied using UV-Visible spectrometry and optical rotation measurement. The results showed that the interaction between BSA and Ni ion comprised of two consecutive reactions. The first reaction was a fast coordination reaction between Ni ion and BSA; the second reaction was the conformational transition of BSA induced by Ni ion. The process was not a simple first order reaction reported by previous papers but an opposite reaction, which comprised of a forward first order reaction and reversible first order reaction.