一种改良的提取草莓属叶片总RNA的方法

An Improved Method for Isolation of Total RNA from the Leaves of Fragaria spp.

探索一种新的适合草莓属(Fragariaspp.)组培苗、大田苗叶片总RNA的快速高效提取方法。试验以草莓叶片为材料,用改进的CTAB法对其RNA进行提取,并对提取的RNA进行了电泳检测、含量测定和RT-PCR检测。结果表明:用该RNA提取方法提取的RNA具有28 S rRNA、18 S rRNA和5.8 S rRNA 3条清晰的条带,且无降解。OD260/OD280在1.83至2.14之间,具有较高的纯度,且含量较高。用该RNA提取方法提取的RNA逆转录成cDNA,经PCR扩增出现清晰的条带,说明该RNA提取方法提取的RNA可以满足进一步分子生物学试验的要求。

A fast and efficient method for isolation of total RNA from the leaves of strawberry （ Fragaria spp. ） in vitro and field was developed in the experiment. The RNA samples of the leaves of strawberry were isolated by improved CTAB method, and the purity and quantity of each RNA obtained was evaluated by agarose gel electrophoresis, UV scanning and RT-PCR analysis. The total RNA of strawberry leaves extracted by this method showed clear bands of 28 S rRNA, 18 S rRNA and 5.8 S rRNA, and the values of OD260/OD280 were between 1.83 and 2. 14. The cDNA reversely transcripted from the extracted RNA exhibited clear band by RT-PCR, which meant that the quality and purity of the RNA could meet the demands of molecular biology experiment.