木菠萝ISSR反应体系的建立和优化

Establishment and Optimization of an ISSR Reaction System in Jackfruit

以木菠萝为材料,研究了模板DNA、Taq DNA聚合酶、引物、Mg2+及dNTPs浓度对ISSR-PCR扩增结果的影响,得出木菠萝ISSR的较佳反应体系为:在20μl反应体系中DNA模板1.6ng.μl-1,Taq DNA聚合酶1.25U.(20μl)-1,引物0.24μmol.L-1,dNTPs0.2mmol.L-1,Mg2+2mmol.L-1,10×PCR缓冲液2.0μl。

Jackfruit was used to study the effects of some influencing factors on ISSR - PCR analysis, such as template DNA, Taq polymerase, primer, dNTPs and Mg^2 ＋. The optimal ISSR - PCR reaction system for Jackfruit was determined as follows： 1.6 ng ·μl^-1 template DNA , 1.25 U · （20μl） ^-1 Taq potymerase, 0. 24 μmol · L^-1 primer, 0.2 mmol · L-1 dNTPs, 2 mmol·L^-1 Mg^2＋ and 2.0 μl 10 × buffer in 20μl PCR reaction system.