分蘖角度动态型水稻的特征与基因定位研究

Gene mapping and character of a rice with dynamic tiller angle

利用一份从贵州引进的分蘖角度在分蘖期半散生、成熟后生长紧凑的水稻特异材料N360,全生育期直立型材料9308和对照材料蜀恢527为研究对象,调查3份材料全生育期分蘖角度的变化,结果表明:从分蘖盛期到成熟期,N360、蜀恢527和9308分蘖角度减小度数分别为30、14和7,°N360具有典型的分蘖角度动态变化特点;将N360与9308杂交,构建F2代定位群体,测量亲本和F2代定位群体分蘖角度的动态变化,采用极端集团-隐性群法,并利用SSR分子标记,初步定位了控制分蘖角度动态型变化的一个主效基因于第9染色体上SSR分子标记RM201和RM215之间,遗传距离分别为21.6和19.0 cM。此外,讨论了这种水稻分蘖角度动态变化性状在育种上的应用。

In this study, changes of tiller angle were studied using three varieties differed in tiller angle （ N360 ： a rice material with semi-scat- tering at the tiller stage and erecting at the full heading stage was introduced from Guizhou province ;9308 ：erecting in full stage;Shuhui 527： aoriginal line） as materials. The results indicated that the decrease degrees of tiller angle of N360, Shuhui 527 and 9308 from the super tiller stage to the maturity stage were 30,14 and 7 degrees, so N360 was a model rice variety with dynamic tiller angle. Using the F2 population from the cross between N360 and 9308 ,the dynamic variation degree of rice tiller angle in parents and F2 population was measured. At the same time, with bulked extremes and recessive class, SSR molecular marker, a major gene with controlling this character was located between SSR marker RM201 and RM215 on chromosome 9 ,with genetic distance of 21.6 and 19.0 cM ,respectively. Moreover,the application of the dynamic tiller angle in rice breeding was also discussed.