过量表达OsNPR1基因稳定提高水稻对白叶枯病的抗性

Over-expression of OsNPR1 in rice stably enhances the rice resistance to bacterial blight

在拟南芥中,NPR1是系统获得抗性SA信号传导途径中的一个重要的调节因子,在水稻中已克隆到与之同源的OsNPR1基因。构建OsNPR1基因水稻过量表达载体,并将其转化粳稻TP309得到转基因植株;通过自交纯合,得到17个纯合株系;对T3、T4代纯合株系进行PCR鉴定,证实转基因纯合株系中外源OsNPR1基因具有遗传稳定性;检测了T1、T2代转基因株系和T3代转基因纯合株系对水稻白叶枯病病原细菌Xanthomonas oryzaepv.oryzae的抗病性,结果表明,在T1、T2代中70%以上的株系对水稻白叶枯病的抗性显著提高,T3代中约67%的株系对水稻白叶枯病的抗性显著提高,说明这种抗病性的提高具有遗传稳定性。OsNPR1基因可作为选育水稻抗白叶枯病新种质的一个良好的候选基因。

NPR1 is a key regulator of the SA-mediated SAR pathway in Arabidopsis thaliana. In rice, an A. thaliana NPR1 homologous gene, OsNPR1, was isolated. In this study, an OsNPR1 rice over-expression vector was constructed and transformed into rice cultivar TP309 and 17 homozygous lines were obtained by self-fertilization and selection. The genetic stability of introduced OsNPR1 in the T3, T4 progenies of homozygous lines was confirmed by PCR. When the transgenic plants were challenged with bacterial blight pathogen Xanthomonas oryzae pv. oryzae, it was found that over 70% of the transgenic lines in T1, T2 progenies and about 67% of homozygous lines in T3 progeny showed significantly enhanced resistance to this disease, indicating that the enhanced resistance of the transgenic rice was stably inherited. The OsNPR1 gene may be used as a good candidate gene in breeding rice for new rice varieties with resistance to bacterial blight disease.