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新型含氟细胞钙离子荧光探针Fluo-3F的合成 被引量：1

Synthesis of novel fluorinated calcium fluorescence probe fluo-3F
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摘要 目的设计并合成新型含氟荧光素类细胞钙离子荧光探针Fluo-3F。方法以5-氟邻硝基苯酚为起始原料,5步反应得到中间体BAPTA-F-CHO,再与4-氯间苯二酚缩合。结果新型含氟荧光素类钙离的结构经核磁共振光谱,红外光谱,质谱,元素分析得到证实。结论合成的新型含氟荧光素类钙离子荧光探针Fluo-3F荧光性能优异,为合成新型钙离子荧光探针提供了新的思路。 Aim To synthesize a novel fluorinated fluorescein-base Ca^2＋ fluorescence probe Fluo-3F. Methods BAPTA-F-CHO was prepared through five steps, then it was reacted with 4-chlororesorcin to give the novel Ca^2＋ fluorescence probe Fluo-3F. Results By ^1H NMR, IR spectra, MS, elemental analysis, the structure of Fluo-3F was confirmed. Conclusion This method provides a new idea for syntheses novel calcium fluorescence probes.
作者 韩洁 李剑利 孙伟 雷磊 史真
机构地区 西北大学化学系
出处 《西北大学学报（自然科学版）》 CAS CSCD 北大核心 2008年第6期947-950,共4页 Journal of Northwest University（Natural Science Edition）
基金 国家自然科学基金资助项目(20472067) 高等学校博士学科点专项科研基金资助项目(20050697003) 西安市科技计划基金资助项目(YF07070)
关键词 钙离子荧光探针 含氟化合物 合成 荧光光谱 calcium fluorescence probe fluorinated compound synthesis fluorescence spectrum
分类号 O625 [理学—有机化学]
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参考文献9

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同被引文献18

  • 1Vendrell M. Combinatorial Strategies in Fluorescent Probe Development [J]. Chemical Reviews, 2012, 112(8): 4391-4420.
  • 2Goslinski T, J Piskorz. Fluorinated porphyrinoids and their biomedical applications [J]. Journal of Photochemistry and Photobiology C: Photochemistry Reviews, 2011, 12(4): 304-321.
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  • 4Rurack K. Chalcone-Analogue Dyes Emitting in tle Near-Infrared (N1R): Influence of Donor-Acceptor Substitution and Cation Complexation on Their Spectroscopic Properties and X-ray Structure [J]. The Journal of Physical Chemistry A, 2000, 104(14): 3087-3109.
  • 5Rurack K. Substituted 1,5-Diphenyl-3-benzothiazol-2-yl-A2-pyrazolines: Synthesis, X-ray Structure, Photophysics, and Cation Complexation Properties [J]. The Journal of Physical Chemistry A, 2000, 104(26): 6171-6188.
  • 6Rurack K. A Selective and Sensitive Fluoroionophore for Hgll, Agl, and Cull with Virtually Decoupled Fluorophore and Receptor Units [J]. Journal of the American Chemical Society, 2000, 122(5): 968-969.
  • 7Yuan M. A Multianalyte Chemosensor on a Single Molecule: Promising Structure for an Integrated Logic Gate [J]. The Journal of Organic Chemistry, 2008, 73(13): 5008-5014.
  • 8Gee K R. Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes [J]. Cell Calcium, 2000, 27(2): 97-106.
  • 9Balcerczyk A M . Soszynski .- On the specificity of 4-amino-5-methylamino-2',7'-difluorofluoreseein as a probe for nitric oxide. Free Radical Biology and Medicine [J]. 2005, 39(3): 327-335.
  • 10Zheng H. Fluorogenic and Chromogenic Rhodamine Spirolactam Based Probe for Nitric Oxide by Spiro Ring Opening Reaction [J]. Organic Letters, 2008, 10(12): 2357-2360.

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西北大学学报（自然科学版）
2008年 第6期

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