红松仁不饱和脂肪酸对肥胖大鼠肝脏脂肪代谢的影响

EFFECTS OF UNSATURATED FATTY ACIDS OF PINUS KORAIENSIS ON LIPID METABOLISM AND BRAIN FUNCTION IN OBESE RATS

目的研究红松仁不饱和脂肪酸对大鼠脂肪代谢的调节作用。方法采用从超临界法提取的红松仁油为原料,用尿素包合法制得不饱和脂肪酸。将大鼠随机分为8组,除1组为基础对照组外,其他7组给予高脂饲料,每周称重一次,建立肥胖模型。基础对照组和肥胖对照组分别灌胃生理盐水(1.2g/kgbw),松仁原油对照组灌胃松仁油(1.2g/kgbw),5个剂量组分别灌胃不饱和脂肪酸0.4、0.8、1.2、1.6和2.0g/kgbw。连续4w后,分别测定大鼠体重、脂/体质量比、血清总胆固醇(TC)、血清总甘油三酯(TG)、高密度脂蛋白(HDL-C)、肝脂酶(HL)活性、脂蛋白脂酶(LPL)活性及肝脏脂褐质(LP)含量。结果与肥胖对照组相比,红松仁不饱和脂肪酸能显著降低大鼠体重、脂体比、TG、LP和TC水平,而HL水平和总脂酶(LA)水平显著提高。结论红松仁不饱和脂肪酸对大鼠脂代谢具有积极的调节作用。

Objective To study the effect of unsaturated fatty acid of Pinus koraiensis on lipid metabolism in rats. Method Unsaturated fatty acid was extracted and concentrated from Pinus koraiensis by supercritical fluid extraction and urea inclusion method. 40 rats were divided into 8 groups randomly. Except normal control group, the other groups were fed high fat diet. Normal control and obesity control groups were fed i.g. normal saline, Seed oil control group was fed i.g. pine nut oil and the other groups were fed i.g. 0.4, 0.8, 1.2, 1.6 and 2.0 g/kg bw unsaturated fatty acid. Body weights were weighed every week.After 4 w, the rats were killed, the serum total triglycerides （TG） , total cholesterol （TC） , high density lipoprotein cholesterol （HDL-C） and hepatic lipase （HL） , lipoprotein lipase （LPL） , liver lipofuscin （LP） were assayed. Results Compared with obesity control group, the body weight, body fat ratio, serum TG, LP and TC level were reduced remarkably. HL and total lipase （LA） level enhanced remarkably. Conclusion The unsaturated fatty acid of Pinus koraiensis could enhance the lipid metabolism in obese rats.