低聚木糖对摄食高脂饲料的小鼠自由基水平及抗氧化作用的影响

EFFECTS OF XYLO-OLIGOSACCHARIDES ON THE LEVEL OF FREE RADICALS AND ANTIOXIDANT CAPACITY OF RATS FED HIGH-FAT DIETS

目的研究高脂饲料条件下,不添加和添加中、高剂量(0.0835%和0.1650%)的低聚木糖(XOS)对小鼠体内自由基及抗氧化功能的影响。方法将24只小鼠分成4组,设置对照,通过向高脂饲料中添加XOS,观察对小鼠机体自由基及抗氧化功能的影响。结果与正常对照组相比,高脂饲料可导致小鼠氧化应激,升高体内自由基水平,降低机体抗氧化能力,而添加XOS的饲料能显著降低机体自由基水平,增强血清及组织中SOD、GSH-Px及CAT活性,显著升高血清T-AOC水平,显著降低血清MDA含量。并且,XOS添加量与抗氧化作用呈现量效关系。结论低聚木糖具有较好的清除机体自由基及抗氧化功能。

Objective To evaluate the effects of xylo-oligosaccharides （XOS） on antioxidative capacity in rats fed with high-fat diet. Method Twenty four rats were randomly divided into 4 groups： control group, high-fat group, high fat with 0.0835% XOS group, high fat with 0.1650% XOS group. Then the levels of free radicals , MDA, T-AOC and the activities of the antioxidant enzymes （SOD, GSH-Px, CAT） were determined at the end of 6 w. Results High-fat diet could induce oxidative stress in rats and increase the level of free radicals. Addition of XOS in high-fat diets could decrease the level of free radicals and enhance the activities of SOD, GSH-Px, CAT in serum and tissue, and increase the level of T-AOC and decrease the content of serum MDA significantly. Moreover, the antioxidant activity was linear with the dosage of XOS in the diets. Conclusion XOS can scavenge free radicals and increase the antioxidative capacity in rats fed with high diet.