籼稻半矮秆品种对GA_3、ABA的敏感性及其与过氧化物酶的关系

SENSITIVITY OF SEMIDWARF GENES TO GA 3, ABA AND THEIR RELATIONSHIP WITH PEROXIDASE IN INDICA RICE

以高秆籼稻品种南京６号（对照）、含不同半矮秆主基因的籼稻品种南京１１、南充一枝腊、新泰矮、新桂矮、双矮为材料，研究了不同半矮秆主基因对ＧＡ３、ＡＢＡ的敏感性及其与过氧化物酶的关系。半矮秆品种第１叶鞘对ＧＡ３的敏感性明显强于苗高对ＧＡ３的反应，随着处理时间的延长，幼苗对ＧＡ３的反应加强，半矮秆基因在苗期对ＧＡ３的敏感性强弱顺序为：ｓｄ－１＞ｓｄ－ｎ（ｔ）＞ｓｄ－ｔ（ｔ）≈Ｓｄ－１＞ｓｄ－ｇ。ｓｄ－ｇ基因在ｓｄ－１对ＧＡ３的反应中有抑制作用。不同半矮秆基因对ＡＢＡ的敏感性有差异，但差异不大，其中ｓｄ－ｇ对ＡＢＡ较敏感。ＧＡ３、ＡＢＡ对不同半矮秆基因的拮抗效应不同，对ＧＡ３１０ｍｇ／ｋｇ＋ＡＢＡ５ｍｇ／ｋｇ敏感性强弱顺序为ｓｄ－ｇ＞ｓｄ－ｎ（ｔ）＞ｓｄ－１ｓｄ－ｇ＞ｓｄ－１＞ｓｄ－ｔ（ｔ）≈Ｓｄ－１。在ＧＡ３、ＡＢＡ的相互逆转中，ＧＡ３促进效应逐渐大于ＡＢＡ抑制效应。在酶活性方面，５日龄高秆品种幼苗过氧化物酶活性低于矮秆品种，对ＧＡ３反应时过氧化物酶活性均下降，且下降百分率的高低顺序与各品种对ＧＡ３不同敏感性一致；对ＡＢＡ反应时过氧化物酶活性显著提高，对ＡＢＡ强敏感的品种过氧化物酶活力最高，酶活性增加百分率也是最?

Sensitivities to GA 3 and ABA of varieties with different semidwarf gene in indica rice and their relationship with peroxidase were studied using indica rice variety——Nanjing 6 (contrast) and five semidwarf varieties The first sheath of semidwarf variety was much more sensitive to GA 3 than seedling height, the sensitivity to GA 3 of the seedling was enhanced with continuance of GA 3 treatment, and the sensitivities of the semidwaf genes to GA 3 were in the order: sd 1>sd n(t)>sd t(t)≈Sd 1>sd g Sensitivity of sd 1 gene to GA 3 was partially inhibited by gene sd g Sensitivities to ABA of different semidwarf gene had some difference, and sd g was the most sensitive Antagonism of GA 3 and ABA on semidwarf variety was different, and the sensitivities of different semidwarf genes to 10 GA 3+5ABA(GA 3 10 mg/kg+ABA 5 mg/kg) were in the order: sd g>sd n(t)>sd 1 sd g>sd 1>sd t(t)≈Sd 1 Promotive effect of GA 3 was gradually stronger than inhibitory effect of ABA with interaction of GA 3 and ABA Assay of peroxidase showed that the activity of the enzyme was lower in the tall variety than that in the short varieties in seedlings of 5 days The activity of peroxidase of the seedlings decreased when treated with GA 3, and decrease percentage of the enzyme activity was correlated with the sensitivity to GA 3 among various varieties The activity of peroxidase in seedlings significantly increased when treated by ABA The variety which was strong sensitive to ABA had the highest peroxidase activity and increase percentage of the enzyme