卡培他滨促进胃癌细胞凋亡的实验研究

Experimental study on the apoptosis of BGC-823 promoted by capecitabine

【目的】研究卡培他滨促进胃癌细胞凋亡的分子机制。【方法】卡培他滨处理人胃癌细胞BGC-823;用透射电镜和琼脂糖凝胶电泳(DNA Ladder)检测细胞凋亡;RT-PCR方法检测caspase-3 mRNA表达;Western Blotting检测Caspase-3蛋白表达。【结果】不同剂量的卡培他滨均可促进胃癌细胞BGC-823凋亡;中、高浓度卡培他滨处理的胃癌细胞与对照组比较,caspase-3 mRNA及蛋白水平均表达上调(P<0.05)。【结论】卡培他滨可促进胃癌细胞BGC-823凋亡,机制可能是增加caspase-3 mRNA及蛋白表达,增强Caspases级联反应,从而促进凋亡的发生。

[ Objective] To study the molecular mechanism of BGC-823 apoptosis promoted by capecitabine. [Methods] Cell apoptosis was analyzed by TEM and DNA agarose gel electrophoresis. The mRNA and protein expression were detected by RT-PCR （caspase-3）, and Western Blotting （Caspase-3） . [Results] Capecitabine could induce the apoptosis of BGC-823. As compared with control, capecitabine of intermediate and high concentration could raise the expression level of caspase-3 mRNA and protein （ P 〈 0.05）. [ Conclusions] The possible mechanism which capecitabine induces BGC-823 apoptosis is to increase the expression of caspase-3 mRNA and protein, and strengthen Caspases cascade reaction.