黄体酮对脑梗死大鼠血脑屏障的保护作用及可能机制

The protective mechanism of progesterone on blood brain barrier in cerebral ischemia in rats

目的本研究拟观察黄体酮对脑梗死大鼠脑部炎症因子表达,血脑屏障完整性的影响。方法建立MCAO大鼠模型,腹腔注射黄体酮,ELISA法检测脑部炎症因子表达,伊文氏蓝染色检测血脑屏障的破坏。PARP抑制剂PJ34腹腔注射观察对以上检测内容的影响。结果脑梗死后,炎症因子的表达水平明显升高,血脑屏障破坏严重。腹腔注射黄体酮能显著降低炎症因子的表达水平,减轻血脑屏障的破坏通透性。注射肿瘤坏死因子(TNF-α)受体阻断剂PJ34,亦有相同的结果。结论黄体酮可以通过抑制炎症因子的表达减轻脑梗死大鼠血脑屏障的破坏,其对脑梗死大鼠具有明显的神经保护作用。

Objective To investigate the inhibitory effects of progesterone on inflammatory response and its influence on integrity of blood brain barrier after stroke. Methods Progesterone（10mg/kg） was injected intraperitoneally following permanent MCAO of rats once a day, and ELISA was applied to detect the expression of tumor necrosis factor-α （TNF-α）. Blood brain barrier（BBB） permeability was evaluated by detection of extravasated Evans blue dye. The PARP inhibitor PJ34 was also administered intraperitoneally after the onset of ischemia. Animals were killed after ischemia and cerebral tissue removed for analysis. Results TNF-α increased in all infarcted animals. In rats treated with PJ34, the expression level of TNF-α was reduced significantly in comparison with sham group. In addition, progesterone-treated injured rats showed the same results. Conclusion Progesterone inhibits the inflammatory response after stroke and mitigates the severity of brain damage.