引种的不同种源黄花蒿青蒿素含量的研究

Study on Contents of Artemisinin in Artemisia annua L. Introduced from Different Provenances

采用恒温振荡浸提法和柱前衍生紫外检测高效液相色谱法,测定黑龙江省引种的黄花蒿青蒿素含量。结果表明:2007年引自酉阳的黄花蒿(YY-2)含青蒿素质量分数最高为0.668%,药用部分生物量最大的为YY-3(4014.6±420.0)kg/hm2,其主要因素受遗传背景的影响。引种的黄花蒿青蒿素含量虽然下降,但能够达到药用收购标准且生物量很大。引种的黄花蒿具有很强的适应性,可利用黑龙江省生产力差的闲置、废弃土地推广,即可获得可观的经济效益,又避免与传统农作物争地。

Methods of extraction under shaking at constant temperature, postcolumn derivativation, ultraviolet detection and HPLC were used to determine contents of artemisinin in Artemisia annua L. which had been introduced into Heilongjiang Province from different provenances. The results of the study were as follows ： the highest artemisinin mass fraction reached about 0. 668 % in A. annua which was introduced from Youyang （YY-2） to Heilongjiang in 2007, which exhibited the highest medicinal biomass （4014.6 ＋ 420.0 kg/hm^2 ）. Affected mainly by self-provenance, artemisinin content of the indroduced A. annua was decreased. However, it is still up to the purchase standard and possesses a large amount of medicinal biomass. The introduced cuhivars have strong adaptability to the environment of Heilongjiang. By planting A. annua, the large uncuhivated lands in Heilongjiang can be utilized to obtain remarkable economic benefit without the problem of land competition with traditional crops.