冬枣叶片愈伤组织诱导研究

Callus inducement in tissue culture of jujube

组织培养条件下,以冬枣组培苗叶片为试材,分别研究了3种基本培养基、5种生长调节剂、不同暗培养时间和不同硝酸银浓度对叶片愈伤的诱导效果。结果表明,MS和1/2MS培养基中的诱导率高,愈伤的质量好;在这两种培养基中TDZ不论是单独使用还是与IBA混合使用都能得到高质量的愈伤组织并能生成不定芽;光照对叶片愈伤的诱导率无明显影响,但经过14天暗培养诱导出的愈伤组织质地致密,出芽数高;加入硝酸银对愈伤诱导率无影响但能明显促进愈伤出芽。

The leaves of Dongzao jujuber were used for studying the effect in callus inducement. The impact factors included 3 different culture medium, 5 different hormones, different culture time in darkness and different concentration of AgNO3. The results showed that, the rate of inducement was higher in MS and 1/2MS culture medium. In the two medium, TDZ and （TDZ ＋ IBA） were good to induce callus and buds. Light was not an important fact orin inducement, but culture without light about 14 days made the callus compact and good for bourgeoning buds. AgNO2 could accelerate bourgeon buds but not effective in callus inducement.