胶原用量对聚乙烯醇-糖胺聚糖-胶原复合支架性能的影响

Effect of collagen dose on property of polyvinyl alcohol-glycosaminoglycan-collagen scaffold

背景:聚乙烯醇是一种亲水性水凝胶物质,具有与人体组织相近的含水量和较高的机械强度,但细胞亲和性较差;糖胺聚糖和胶原是天然生物材料,能促进细胞生长,但单独使用时,机械性能较差。目的:观察胶原对聚乙烯醇-糖胺聚糖-胶原复合支架性能的影响。设计、时间及地点:多组对照选择,于2007-03/2008-06在广州暨南大学生物材料省重点实验室,教育部再生医学研究中心重点实验室完成不同配比设计的材料对照实验。材料:聚乙烯醇124(广州市医药公司),糖胺聚糖(BSChemical Technology),胶原(广州市红十字会医院创伤外科研究所,广州创尔生物技术有限公司),NaOH为国产分析纯。方法:聚乙烯醇、糖胺聚糖与0,0.01,0.02,0.03,0.04,0.05g胶原复合,制备聚乙烯醇-糖胺聚糖-胶原复合支架,测定复合支架的含水率、孔隙率和透光率。主要观察指标:不同胶原用量对复合支架含水率、孔隙率和透光率的影响。结果:①糖胺聚糖用量为0g和0.01g时,加入胶原,复合支架的含水率升高;而糖胺聚糖用量为0.03,0.05,0.07,0.09g时,含有胶原的复合支架含水率较不含胶原时下降。②含有胶原的复合支架,其孔隙率均比不加胶原时增大。③复合支架的含水率和孔隙率均随胶原用量增加而增加,但孔隙率在胶原用量为0.02g时出现最大值。④胶原的加入使复合支架的透光率下降,并且随着胶原用量的增加,透光率明显减少。结论:通过改变胶原在复合支架中的含量,能制备得到具有较高含水率、适宜孔隙率和透光率的聚乙烯醇-糖胺聚糖-胶原复合支架。

BACKGROUND： Polyvinyl alcohol （PVA） is a hydrophilic polymer, which has an advantage of tissue-like water content and excellent mechanical strength. However, it exhibits poor cell adhesion. As natural biomaterials, glycosarninoglycan （GAG） and collagen （COL） are good at cell adhesion, whereas shows poor mechanical properties when used alone. OBJECTIVE： To investigate the effect of collagen on property of PVA-GAG-COL scaffold. DESIGN, TIME AND SETTING： The comparative experiment among many groups was performed at the Key Laboratory of Biomaterials and Ministry of Education Laboratory for Regenerative Medicine Research Center from March 2007 to June 2008. MATERIALS： PVA-124 （Guangzhou Pharmaceuticals Corporation）, GAG （BS Chemical Technology）, collagen （Guangzhou Institute of Traumatic Surgery, Guangzhou Trauer Biotechnology）, NaOH was analytical reagent. METHODS： Collagen at different dosage of 0, 0.01, 0.02, 0.03, 0.04, and 0.05 g was blended with PVA and GAG to fabricate PVA-GAG-COL composite materials. Water content, porosity and transmittance were tested. MAIN OUTCOME MEASURES： The effect of different dosage of collagen on property and structure of composite materials. RESULTS： When the dosage of GAG was at 0 and 0.01 g, the water content of the scaffolds was increased while collagen was added. When the dosage of GAG was at 0.03, 0.05, 0.07 and 0.09 g, the water content of the scaffolds with collagen was lower than the scaffolds without collagen. The porosity of the scaffold with collagen was higher than the scaffold without collagen. The water content and porosity of scaffold were increased with increasing dosage of collagen. The porosity reached the maximum when the dosage of collagen was 0.02 g. Collagen decreased transmittance of the scaffolds. With dosage increasing of collagen, the transmittance of the scaffolds significantly decreased. CONCLUSION： A scaffold for tissue engineering with high water content and proper porosity and transmittance can be fabricated by changing the dosage of collagen.