猪骨支架材料与人骨支架材料的理化性能及力学特征(英文)

Mechanical and physicochemical properties of xenogeneic bone scaffold materials:A comparative study

背景:骨组织工程研究的核心是构建类似人体骨组织结构和性能的组织工程支架。目的:对比观察猪骨支架材料与人骨支架材料的理化性能及力学性能。设计、时间及地点:对比观察实验,于2006-03/12 在南方医科大学临床解剖学研究所和广东省组织构建与检测重点试验室完成。材料:新鲜健康成人尸体 4 具由广州市红十字会南方医科大学遗体捐献接收点提供,家属知情同意。市售低温深冻 6 个月的成年猪6 只。方法:取人和猪髂骨,剔除软组织,刮除骨髓和骨膜,用锯骨机将松质骨切成 5 mm× 5 mm×40 mm 左右的骨条,超声清洗、H2O2和乙醇浸泡、甩干、冻干、辐照处理得到猪骨支架材料和人骨支架材料。主要观察指标:对两种支架材料进行扫描电镜观察;对比两种支架材料孔隙率、蛋白质和钙、磷含量及力学性能。结果:扫描电镜下两种材料均具有骨本身的骨小梁、小梁间隙及骨内管腔系统,具有天然网状结构。三维支架系统形态完整,其中猪骨支架材料较人骨支架材料具有更多的三维孔隙,2 种材料的孔隙大小接近,均在400 μm左右。猪骨支架材料孔隙率高于人骨支架材料(P < 0.05),蛋白质含量低于人骨支架材料(P < 0.05),钙、磷含量与人骨支架材料相当(P > 0.05)。两种支架材料弹性模量比较差异无显著性意义(P > 0.05)。结论:猪骨支架材料在理化性能和力学性能方面与人骨支架材料极相近。

BACKGROUND： The core of bone tissue engineering is to construct a scaffold that is similar to human bone tissue structure and features. OBJECTIVE： To compare pathochemical and mechanical characteristics between pig and human bone scaffold materials. DESIGN, TIME AND SETTING： contrast study was performed at Clinical Anatomy Institute, South Medical University; Guangdong Province Key Laboratory of Tissue Construction and Detection from March to December 2006. MATERIALS： Four fresh health adult human cadavers were provided by South Medical University, Guangzhou Red Cross Society, and the relatives knew the fact. Ultra low temperature freezing 6-month iliac bones of 6 adult swines were also used in this study. METHODS： Pig iliac and healthy adults iliac bones were obtained to remove soft tissue, curettage periosteum and bone marrow. Bone sawing machine was used to cut cancellous bone into smaller bone sections around 5 mm × 5 mm × 40 mm, which underwent ultrasonic cleaning, H2O2 and alcohol soaking, freeze drying and radiation treatment; finally, xenogeneic bone scaffold and allogeneic bone scaffold were obtained. MAIN OUTCOME MEASURES： Xenogeneic bone scaffold material and human allograft bone scaffold were observed with scanning electron microscopy to compare porosity, contents of protein content, calcium and phosphorus, and mechanical properties. RESULTS： Xenogeneic bone scaffold and allogeneic bone scaffold both had intrinsical bone trabecula, trabecular spaces and bone cavity system. Both of them had unabridged natural three dimensional network structure. The 3D supporting frames of them were complete. The xenogeneic bone scaffold had more spaces than allogeneic bone scaffold. The size of both scaffolds was approximation, about 400 μm. The interval porosity of xenogeneic bone scaffold was higher than the allogeneic bone scaffold （P 〈 0.05）. And the protein of xenogeneic bone scaffold was not as many as it of allogeneic bone scaffold （P 〈 0.05）. The contents of Ca and P were similar （P 〉 0.05）, and there was no significant difference in Young＇s modulus of xenogeneic bone scaffold and allogeneic bone scaffold （P 〉 0.05）. CONCLUSION： Xenogeneic bone scaffold may completely meet the clinical demands for bone grafting or be the scaffold of bone tissue engineering in mechanical chemical properties.