聚己内酯隔离膜对大鼠术后腹腔组织粘连的作用(英文)

Prevention of peritoneal adhesion formation utilizing degradable polycaprolactone film

背景：研究表明，聚己内酯隔离膜对大鼠的心、肝、肾等重要器官细胞免疫功能无明显影响，具备抗粘连隔离物的必要条件。目的：观察聚己内酯隔离膜在预防大鼠术后腹腔粘连中的作用。设计、时间及地点：随机对照动物实验，于200802在吉林大学生理学教研室完成。材料：雄性Wistar大鼠120只，体质量180～220g，用于制备腹腔粘连模型；乳胶隔离膜为武汉博士德生物公司产品；聚己内酯隔离膜为英国U．C．C．公司产品。方法：将120只大鼠按随机编号法分为3组，每组40只。对照组建立腹腔粘连动物模型，乳胶隔离膜组和聚己内酯隔离膜组在造模基础上，分别将乳胶隔离膜和聚己内酯隔离膜固定在腹膜创面上。术后第1天，向腹腔内注射浓度为2g／L的假单胞杆菌脂肪酶溶液，4mL／次，连续3d。主要观察指标：3组分别于造模后l，3，7，30d各取10只大鼠，开腹观察腹腔粘连情况。结果：120只Wistar大鼠均进入结果分析。对照组全部发生腹腔粘连，聚己内酯隔离膜组粘连率术后逐渐降低，至第7天起明显低于对照组（P〈0．01）；乳胶隔离膜组粘连率先降低后又升高，第30天时明显高于聚己内酯隔离膜组（P〈0．01）。结论：聚己内酯隔离膜能被假单胞杆菌脂肪酶降解，避免异物刺激产生新的粘连。

BACKGROUND： Several studies have demonstrated that degradable polycaprolactone film has no apparent influences on cellular immune function of rat critical organs including heart, liver, and kidney, and possesses the prerequisite of anti-adhesion formation. OBJECTIVE： To investigate the role of degradable polycaprolactone film in preventing rat peritoneal adhesion following surgery. DESIGN, TIME AND SETTING： The present randomized, controlled animal experiment was performed at the Department of Physiology, Jilin University. MATERIALS： A total of 120 male Wistar rats, weighing 180-220 g, were included in this study. Peritoneal adhesion was induced in all rats. Non-bio-degradable film was sourced from Wuhan Boster Company, China. Polycaprolactone film was purchased from U.C.C Company, England. METHODS： All 120 rats were randomly divided into 3 groups, with 40 rats per group： control, non-bio-degradable film, and polycaprolactone film. Peritoneal adhesion was induced in all rats. The non-bio-degradable film and polycaprolactone film groups were subjected to peritoneal wound cover with non-bio-degradable film and polycaprolactone film, respectively. From day 1 following surgery, each rat daily received an intraperitoneal injection of 4 mL 2 g/L pseudomonas enzyme, for a total of 3 successive days. MAIN OUTCOME MEASURES： At 1, 3, 7, and 30 days following surgery, 10 rats were selected from each group for observation of peritoneal adhesion. RESULTS： Totally 120 rats were included in the result analysis. Peritoneal adhesion was successfully induced in rats from the control group. From day 7 following surgery, the peritoneal adhesion rate was significantly lower in the polycaprolactone film group than in the control group （P 〈 0.01）. In the non-bio-degradable film group, the peritoneal adhesion rate exhibited decreasing followed by increasing tendency; by day 30, the adhesion rate was significantly higher compared to the polycaprolactone film group （P 〈 0.01）. CONCLUSION： Polycaprolactone film is degraded by pseudomonas enzyme to avoid the new adhesion caused by foreign stimulation.